Reports from Virginia Commonwealth University Advance Knowledge in Sepsis
By a News Reporter-Staff News Editor at Blood Weekly -- Investigators publish new report on Bacterial Infections and Mycoses. According to news reporting originating from Richmond, Virginia, by NewsRx correspondents, research stated, "Neutrophil extracellular trap (NET) formation was recently identified as a novel mechanism to kill pathogens. However, excessive NET formation in sepsis can injure host tissues."
Our news editors obtained a quote from the research from Virginia Commonwealth University, "We have recently shown that parenteral vitamin C (VitC) is protective in sepsis. Whether VitC alters NETosis is unknown. We used Gulo-/-mice as they lack the ability to synthesize VitC. Sepsis was induced by intraperitoneal infusion of a fecal stem solution (abdominal peritonitis, FIP). Some VitC deficient Gulo-/-mice received an infusion of ascorbic acid (AscA, 200 mg/kg) 30 min after induction of FIP. NETosis was assessed histologically and by quantification for circulating free DNA (cf-DNA) in serum. Autophagy, histone citrullination, endoplasmic reticulum (ER) stress, NF?B activation and apoptosis were investigated in peritoneal PMNs. Sepsis produced significant NETs in the lungs of VitC deficient Gulo-/-mice and increased circulating cf-DNA. This was attenuated in the VitC sufficient Gulo-/-mice and in VitC deficient Gulo-/-mice infused with AscA. Polymorphonuclear neutrophils (PMNs) from VitC deficient Gulo-/-mice demonstrated increased activation of ER stress, autophagy, histone citrullination, and NF?B activation, while apoptosis was inhibited. VitC also significantly attenuated PMA induced NETosis in PMNs from healthy human volunteers. Our in vitro and in vivo findings identify VitC as a novel regulator of NET formation in sepsis."
According to the news editors, the research concluded: "This study complements the notion that VitC is protective in sepsis settings."
For more information on this research see: Vitamin C: a novel regulator of neutrophil extracellular trap formation. Nutrients, 2013;5(8):3131-51 (see also Bacterial Infections and Mycoses).
The news editors report that additional information may be obtained by contacting B.M. Mohammed, Dept. of Pharmacotherapy and Outcomes Science, Virginia Commonwealth University, Richmond, VA 23298, United States. Additional authors for this research include B.J. Fisher, D. Kraskauskas, D. Farkas, D.F. Brophy, A.A. Fowler and R. Natarajan.
Keywords for this news article include: Sepsis, Richmond, Virginia, Immunology, Phagocytes, Septicemia, Blood Cells, Neutrophils, Granulocytes, United States, Bloodstream Infection, Hemic and Immune Systems, North and Central America, Bacterial Infections and Mycoses.
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