New Liver Diseases Study Findings Have Been Reported by Researchers at Institute for Chemistry (Coffee enhances the expression of chaperones and antioxidant proteins in rats with nonalcoholic fatty liver disease)
By a News Reporter-Staff News Editor at Gastroenterology Week -- Investigators discuss new findings in Liver Diseases. According to news originating from Catania, Italy, by NewsRx correspondents, research stated, "Coffee consumption is inversely related to the degree of liver injury in patients with nonalcoholic fatty liver disease (NAFLD). Molecular mediators contributing to coffee's beneficial effects in NAFLD remain to be elucidated."
Our news journalists obtained a quote from the research from Institute for Chemistry, "In this study, we administrated decaffeinated espresso coffee or vehicle to rats fed an high-fat diet (HFD) for 12 weeks and examined the effects of coffee on liver injury by using two-dimensional polyacrylamide gel electrophoresis (2D-PAGE) proteomic analysis combined with mass spectrometry. Rats fed an HFD and water developed panacinar steatosis, lobular inflammation, and mild fibrosis, whereas rats fed an HFD and coffee exhibited only mild steatosis. Coffee consumption increased liver expression of the endoplasmic reticulum chaperones glucose-related protein 78 and protein disulfide-isomerase A3; similarly, coffee drinking enhanced the expression of the mitochondrial chaperones heat stress protein 70 and DJ-1. Furthermore, in agreement with reduced hepatic levels of 8-isoprostanes and 8-hydroxy-2'-deoxyguanosine, proteomic analysis showed that coffee consumption induces the expression of master regulators of redox status (i.e., peroxiredoxin 1, glutathione S-transferase 2, and D-dopachrome tautomerase). Last, proteomics revealed an association of coffee intake with decreased expression of electron transfer flavoprotein subunit, a component of the mitochondrial respiratory chain, involved in de nova lipogenesis."
According to the news editors, the research concluded: "In this study, we were able to identify by proteomic analysis the stress proteins mediating the antioxidant effects of coffee; moreover, we establish for the first time the contribution of specific coffee-induced endoplasmic reticulum and mitochondrial chaperones ensuring correct protein folding and degradation in the liver."
For more information on this research see: Coffee enhances the expression of chaperones and antioxidant proteins in rats with nonalcoholic fatty liver disease. Translational Research, 2014;163(6):593-602. Translational Research can be contacted at: Elsevier Science Inc, 360 Park Ave South, New York, NY 10010-1710, USA. (The Endocrine Society - www.endo-society.org/; Translational Research - www.endojournals.org/site/trem/)
The news correspondents report that additional information may be obtained from F. Salomone, CNR, Inst Chem & Technol Polymers, Catania, Italy. Additional authors for this research include G.L. Volti, P. Vitaglione, F. Morisco, V. Fogliano, A. Zappala, A. Palmigiano, D. Garozzo, N. Caporaso, G. D'Argenio and F. Galvano (see also Liver Diseases).
Keywords for this news article include: Antioxidants, Italy, Europe, Catania, Proteomics, Protective Agents, Fatty Liver Disease, Digestive System Diseases
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