Life Extension Magazine®

Protecting Against Glycation and High Blood Sugar with Benfotiamine

Lifelong exposure to even moderate blood glucose levels induces systemic damage throughout the body. A supplement widely used by European doctors has been found to block damage caused by chronic glucose exposure to nerves and small blood vessels.

Scientifically reviewed by: Dr. Gary Gonzalez, MD, in August 2023. Written by: Julius G. Goepp, MD.

Protecting Against Glycation and High Blood Sugar with Benfotiamine

For decades, European doctors have prescribed diabetic patients a fat-soluble form of vitamin B1 called benfotiamine to treat neuropathies and help prevent complications such as blindness, kidney failure, heart attack, and limb amputation.

Benfotiamine blocks destructive biochemical pathways that enable high blood sugar levels to damage nerves and small blood vessels.

Benfotiamine also inhibits the formation of advanced glycation end products in both diabetic and normal aging organisms. Glycation not only causes kidney, nerve, and retinal damage in diabetics, but is also a significant contributory factor in cardiovascular disease and other aging disorders in adults without diabetes.1-6

Here, we’ll explore how to use benfotiamine to help protect against the dangers of elevated blood sugar and toxic glycation reactions.

AGE, RAGE, and Tissue Damage

Sugar molecules, in excess quantity or over long periods of time, wreak havoc on human tissues. As the number of people with type 2 diabetes has skyrocketed over the past 20 years, we’ve learned to recognize strong relationships between blood sugar levels and many chronic conditions previously thought to be purely age-related. The link seems to be the total lifetime exposure to blood sugar that’s the culprit. In other words, diabetics develop problems sooner because of their chronic higher sugar levels, but even non-diabetic people eventually suffer tissue injury related to the interaction between blood sugar and tissue proteins.

Studies of diabetic patients have shown that prolonged tissue exposure to elevated glucose levels results in the production of a class of molecules called “advanced glycation end products” (AGEs).1-4 These molecules are proteins and lipids that have become bonded to some of the sugar molecules found in high concentrations in diabetics’ blood.5 Advanced glycation end products are well known for their destructive activities in diabetes, where they contribute to vascular disease, kidney failure, eye damage, and other kinds of dysfunction,5,6 including the nerve damage known as diabetic peripheral neuropathy.3,4,7 In patients with this kind of neuropathy, AGEs directly damage vital components of nerve cells,8 insidiously reducing their ability to conduct warning sensations such as pain and pressure.

AGE, RAGE, and Tissue Damage

People with peripheral neuropathies may not perceive the need to shift their weight, for example, to allow an even distribution of blood flow to a pressure point such as the buttock or foot.9 Inadequate blood flow to such areas in turn can result in tissue death (necrosis), producing pressure sores that invite infection.10 And because AGEs also impair immune function, those infections in vulnerable diabetic patients can rapidly become life- and limb-threatening; in fact, amputation of an entire extremity can often be the only treatment if the condition cannot be prevented (foot infections are the single most common cause of hospitalization and amputations in persons with diabetes).11

Our rapidly expanding experience with the diabetic population has taught us that the AGE-related damage to tissues involves a specific chemical receptor that responds to the presence of AGE molecules by inducing inflammation. That receptor molecule is appropriately termed RAGE (for “receptor for AGE”).8

And we’re learning that it’s not just diabetics who are affected—with enough time and exposure, we all develop “AGE-related” problems.6 Ominously, AGE/RAGE reactions occur even in people without known diabetes or insulin resistance, although those conditions hasten the process.12 With advancing age, even normal blood sugar levels, given sufficient time, produce AGEs that act on RAGEs to induce deadly inflammation in blood vessels, nerves, liver, and other vital tissues.6,13,14 A growing number of studies are now demonstrating links between levels of AGEs in the blood of non-diabetics and major risk factors for atherosclerosis and heart disease.15,16

Credible scientists have even speculated that these AGE-related changes could ultimately contribute to an “upper limit” of the human life span17—with the implication that if we could prevent them from happening, we could conceivably extend human life expectancy dramatically!

Thiamine—The Natural Anti-AGE/RAGE Vitamin

One of the most important natural anti-AGE compounds is vitamin B1, or thiamine, and its natural derivative, benfotiamine. Thiamine is a well-known nutrient that has myriad roles throughout the body, particularly in maintaining the healthy, normal functioning of nerve cells.18 Thiamine has been shown to interfere with the AGE/RAGE process in meaningful ways.19,20

Thiamine—The Natural Anti-AGE/RAGE Vitamin

A vivid demonstration of thiamine’s ability to block AGE production and its effects in the laboratory was provided in 1996 by researchers in Italy, who subjected cells in culture to high glucose levels both with and without thiamine supplementation.21 The researchers measured the cells’ ability to function normally and also their production of AGE molecules. High glucose inhibited normal cell reproduction and metabolic activity—but the addition of thiamine significantly reduced total AGE formation, increased cell survival, and restored reproductive activity to normal levels.21 These findings and those of other laboratory investigators have led to calls for trials of high-dose thiamine to prevent glycation-induced damage to nerve cells8 and other tissues such as the liver, where AGE-induced damage plays a key role in the development of cirrhosis.5,22

Scientists in Toronto were able to prevent glycation damage to liver cells in culture by supplementing them with thiamine.23 They also showed that when liver cells were made thiamine-deficient, they were susceptible to damage at much lower levels of glycation products than were normal cells, which suggests that avoiding thiamine deficiency might be an important cirrhosis-prevention strategy.

And Indian researchers recently demonstrated the potential anticancer activity of thiamine.24 They discovered thiamine to be one of the B vitamins that blocks the production of free radicals by AGE molecules, which in turn prevents dangerous DNA injury that can lead to cancer initiation.

These promising results with thiamine supplementation led to the natural question: why not simply use high-dose thiamine to prevent AGE formation in diabetics, and perhaps even in non-diabetic people as well? The answer is based on the concept of bioavailability—not all beneficial molecules are equally well absorbed from the digestive tract, and once absorbed, they may be extensively metabolized and/or poorly penetrate target tissues. Water-soluble nutrients like thiamine are well absorbed, but tend not to penetrate lipids to any great degree (oil and water don’t mix well)—so they aren’t as bioavailable to target tissues that are composed of lipids (fats). This is in part because cell membranes themselves are primarily made up of lipid molecules.

Thus, as a water-soluble vitamin, thiamine has an upper dose limit beyond which additional quantities are simply excreted in urine.25,26 Clearly, for the beneficial anti-AGE effects and related reduction in inflammatory markers to be realized, a better means of delivering thiamine to tissues is needed. Fortunately, thiamine’s fat-soluble cousin benfotiamine is a highly bioavailable nutrient that achieves better distribution throughout the body.27,28

What You Need to Know: Benfotiamine
  • Advanced glycation end products (AGEs) are produced in most tissues exposed to elevated blood sugar levels, and even in tissues exposed to normal blood sugar for a long enough time.

  • Advanced glycation end products induce oxidative stress and inflammation, and are responsible for a large amount of tissue damage and dysfunction in diabetic patients. They are increasingly being recognized as harmful in non-diabetic people as well.

  • The fat-soluble thiamine-derived nutrient benfotiamine blocks three of the major biochemical pathways through which high blood sugar promotes tissues damage—without known side effects. Benfotiamine can reach tissues more than five times more readily than thiamine (vitamin B1) itself, and has been widely used in Europe for decades.

  • An explosion of laboratory and human data now demonstrate that benfotiamine can block the actual tissue-level effects of AGEs and prevent their potentially deadly consequences in both diabetic and non-diabetic people.

Benfotiamine: A Supercharged Thiamine Derivative

Benfotiamine is better than thiamine at penetrating cell membranes and protecting AGE-vulnerable tissues from harmful glucose-protein and glucose-lipid reactions, helping to protect nerve, retinal, kidney, and other cells. Studies suggest that the benefits of benfotiamine may greatly outpace those of thiamine. Biochemical nutritionists at the Friedrich Schiller University in Germany studied three thiamine preparations in seven healthy volunteers and evaluated the bioavailability of each compound.29 Subjects took 100 mg of either benfotiamine, thiamine disulfide, or fursultiamin (another thiamine derivative), and their blood levels of thiamine were measured repeatedly over a 10-hour period, along with biochemical markers of whether cells were actually “seeing” the effects of thiamine. Benfotiamine produced a more rapid and earlier increase in blood and displayed significantly increased bioavailability, compared with either of the other two formulations. These researchers concluded that benfotiamine is the best form of thiamine for therapeutic use.

Benfotiamine: A Supercharged Thiamine Derivative

The same researchers found similar results in a more detailed study of benfotiamine versus thiamine mononitrate in patients with end-stage renal disease (who are frequently thiamine-deficient).30 Subjects who received 100 mg/day of benfotiamine had faster and higher peak levels than those receiving standard thiamine supplements, achieving nearly double the levels and allowing an amazing 420% increase in total 24-hour tissue exposure after a single dose. These results were interpreted as further justification for the use of benfotiamine in patients who require high intracellular concentrations of thiamine. Later studies from the same group demonstrated that the elevated tissue levels produced by benfotiamine markedly increased the all-important cellular content,31 providing conclusive evidence of benfotiamine’s overall superiority to thiamine in blocking formation of AGEs and the RAGE reactions that follow. Benfotiamine’s anti-AGE activity has now been shown to be superior to that of thiamine alone in the treatment of neurodegenerative diseases and cancer,32 as well as on the kidney, eye, and nerve damage seen in diabetic patients.4

Benfotiamine—Combating AGE and RAGE

It is clear that benfotiamine can reach AGE-vulnerable tissues and cells at high levels. In looking at how benfotiamine performs at actually preventing the AGE/RAGE reactions from taking place, Italian scientists collaborating with the World Health Organization studied its effects in correcting endothelial cell damage caused by elevated glucose levels33—just the kind of damage we know is caused by AGEs in both diabetic and non-diabetic people. The researchers cultured human endothelial cells in normal and very high glucose concentrations, treating them with benfotiamine or thiamine, and measuring the cells’ survival and rates of healthy reproduction. The high glucose levels predictably impaired cell replication by nearly 30%—and addition of thiamine raised survival to 80% of normal, while benfotiamine raised it to nearly 90%! Benfotiamine also cut AGE production in the high-glucose environment from 160% of normal to similar levels found in healthy glucose-exposed cells. The scientists speculated that these dramatic effects resulted from benfotiamine’s ability to help cells use glucose more efficiently, reducing the level of waste products that are most active in producing AGEs.

Preventing Diabetic Complications in Animals

Studies in animals powerfully support these encouraging findings with research from a number of quarters revealing the benefits of benfotiamine in preventing diabetic complications. German researchers working with diabetic rats studied the effects of benfotiamine and thiamine on nerve function and on the formation of AGEs in nerve tissue.28 In one group of animals, they studied the preventive effects of the supplements, administering them immediately after diabetes was chemically induced. A second group of animals received supplements only two months later, to study the supplements’ treatment effects. As expected, nerve conduction speeds dropped off in diabetic animals, while AGE levels increased by up to five-fold. In the prevention group, nerve conduction increased substantially compared with control animals, and was nearly normal by six months in the benfotiamine-supplemented group (no further improvement was seen after three months in the thiamine-supplemented animals). Remarkably, similar results were also found in the benfotiamine treatment group—but not in the thiamine-treated animals. Benfotiamine also dramatically reduced AGE formation in nerve cells, which probably explains its dramatic effects. These researchers concluded that “timely administration of benfotiamine was effective in prevention of functional damage and of AGE formation in nerves of diabetic rats”—a radically promising finding!

Preventing Diabetic Complications in Animals

In 2003, British researchers treated rats with benfotiamine and thiamine aimed at preventing diabetic nephropathy (kidney disease), a condition that is associated with a very high risk of cardiovascular disease and death.34 They found that high-dose therapy with benfotiamine and thiamine prevented the accumulation of molecules that trigger the formation of AGEs, oxidative stress, and inflammation—the major factors that ultimately produce kidney damage. They also found that kidney function actually improved in the animals treated with benfotiamine and thiamine, demonstrating not only biochemical but actual whole-organism benefits.

In related studies, German and American physicians showed that treating diabetic rats with high doses of benfotiamine prevented retinopathy, another complication of diabetes.35 The researchers pointed out that benfotiamine works by simultaneously inhibiting three major pathways by which high sugar levels cause tissue damage, such as retinopathy.36 Benfotiamine was also shown to stimulate a key enzyme called transkelotase, which converts potentially damaging glucose breakdown products into harmless compounds that are safely eliminated. Together, these actions make benfotiamine a front-runner in preventing the development of diabetic complications.35,36

Another important and life-threatening complication of diabetes is the heart condition known as diabetic cardiomyopathy, in which individual heart muscle cells cannot contract as strongly as they normally would. The reasons for these changes are only partially understood, but appear to be related both to oxidative stress and inflammation at least in part caused by AGEs affecting the contractile proteins that cause heart muscle cells to beat.37-40

Cardiovascular and alternative medicine researchers at the University of Wyoming put these ideas to the test, treating mice with benfotiamine therapy 14 days after inducing diabetes in the animals. They then studied characteristic features of heart muscle performance that indicated how strongly the hearts were beating and how well the heart tissue responded to chemical signals. Astonishingly, benfotiamine treatment abolished the muscle cell abnormalities induced by diabetes in these animals! Oxidative stress was also measurably reduced though in this short-term study, the researchers did not find effects on AGEs.41

Aging and AGEs

Bizarrely, AGEs are formed by the interaction of glucose and proteins through a specific kind of chemical reaction, called the “Maillard” reaction, that is identical to the so-called “browning reactions” found in ripening fruits and foods cooked at high temperatures.58,59 Maillard reactions are distinctly unwelcome in human tissues, where their AGE-related products produce powerfully destructive oxidative stress and inflammatory reactions,6,15,19 which ultimately lead to atherosclerosis and its deadly consequences.6,15 By “cross-linking” with collagen and other long-lived proteins, AGEs have far-reaching deleterious effects by reducing the elasticity of tissues such as blood vessels where these proteins reside.60 Furthermore, the biochemical changes induced by AGEs may also damage DNA,24 which, together with inflammation, can promote the development of cancer.57

Clearly, the Maillard “browning” reactions occur all the time in our bodies, gradually adding to our burden of oxidative stress, inflammation, and tissue dysfunction—and ultimately to our cumulative risk of serious chronic age-related diseases.

Further research has also shown that benfotiamine is effective at preventing slow-to-heal pressure sores, which are a major cause of suffering and even death in diabetic patients. A group of researchers in Italy used benfotiamine to speed the healing of such wounds.42 Diabetic mice were given oral benfotiamine or a control liquid, and then underwent ischemia (restricted blood flow) of one limb. The scientists then measured tissue indicators of cell death and destruction. Remarkably, benfotiamine completely prevented ischemia-induced necrosis (tissue death) in animals’ toes, improved blood flow and oxygenation, and restored normal blood vessel relaxation (an important control mechanism that is lost in AGE-induced endothelial injury). But that’s not all—closer microscopic examination of the tissue showed substantially improved new blood vessel formation and inhibition of cell death in the affected limbs. Supplementation also prevented accumulation of AGEs in the blood vessels, which probably accounts for the remarkable benefits. The authors were understandably exuberant in their concluding statement, “We have demonstrated, for the first time, that benfotiamine aids the post-ischemic healing of diabetic animals…”

An explosion of convincing animal studies further supports benfotiamine’s remarkable benefits. Benfotiamine has now been shown to counteract AGE-related toxicity on endothelial cells,43 to relieve inflammatory and neuropathic (nerve-induced) pain in both diabetic and non-diabetic rats,44 and to alleviate diabetes-induced oxidative damage to brain tissue.45 Interestingly, benfotiamine’s protective effect on brain tissue is produced by mechanisms that seem to be unrelated to AGE formation, opening the door to even greater benefits in this area!

There is clearly no doubt that benfotiamine has proven itself in laboratory studies, and research is now yielding convincing evidence from human trials as well. Dramatic results are flowing in from top-level researchers around the world, demonstrating that benfotiamine’s potent AGE/RAGE inhibition translates directly into measurable benefits for humans.

Preventing Diabetic Complications in Humans

Since benfotiamine has such potent anti-AGE and oxidative stress-reducing effects, scientists wondered if it would be helpful in treatment of diabetic neuropathies in humans. To investigate, German researchers conducted a randomized, double-blind, controlled study of benfotiamine plus vitamins B6 and B12 in patients with diabetic polyneuropathy.46 This study of 24 patients lasted 12 weeks, and demonstrated dramatic improvement in nerve conduction velocity (the speed with which electrical signals move along nerves) in benfotiamine recipients versus controls. There was also an improvement of patients’ ability to sense vibration (again, an important measure of the ability to perceive pressure and motion). None of the patients experienced side effects related to treatment. These researchers concluded that benfotiamine (in combination with the B vitamins) “represents a starting point in treatment of diabetic polyneuropathy.”

Three years later, Hungarian researchers conducted dose-finding studies of benfotiamine. These scientists treated patients who had painful diabetic neuropathy with a higher-dose benfotiamine treatment of 320 mg/day or with medium-dose treatment of 150 mg/day. This was a six-week-long, open trial among 36 subjects ranging from 40 to 70 years old whose diabetes was under reasonably good control. All patients experienced significant improvements in pain and vibration sensation, as well as in objective measurements of nerve function, with most improvements being evident by the third week of the study. The greatest changes were seen in the high-dose recipients. The researchers concluded that benfotiamine is effective even in the medium-dose range, but that maximum benefits would be found with higher doses. These findings represent a major breakthrough in the management of this heartbreaking and deadly complication of diabetes.47

Results such as these have now been confirmed by independent research groups, one of whom studied 40 inpatients with diabetic polyneuropathy of not more than two years’ duration.48 Twenty of their subjects received benfotiamine 100 mg four times daily, while the others got only placebo tablets. The researchers used a standardized neuropathy score that included measurements of vibration perception as well as patients’ and their physicians’ subjective assessments (it’s just as important to know that the patient feels better as it is to know that their “numbers” are improving). Dramatic improvements were seen in benfotiamine patients’ scores compared with controls, with the most pronounced effect being a reduction in pain. More benfotiamine patients than placebo patients also felt that their overall condition improved, and no patients in either group experienced side effects. The authors pointed out that their findings confirmed the results of earlier trials, and provided “further evidence for the beneficial effects of benfotiamine in patients with diabetic neuropathy.”

Nutritional research on benfotiamine has now expanded, with a growing number of studies evaluating its benefits in areas other than diabetic neuropathy. One important area is the prevention of endothelial dysfunction in both large and microscopic blood vessels, a major contributor to atherosclerosis in diabetic as well as non-diabetic patients.

In the first study of its kind, investigators looked at the effects of benfotiamine in blocking endothelial dysfunction and oxidative stress after consuming a meal rich in dietary AGEs.49 They provided type 2 diabetic subjects with a heat-processed test meal that had a high AGE content, both before and after a three-day period of supplementation with 1,050 mg/day of benfotiamine. The scientists measured standard indicators of large- and small-vessel function, as well as blood levels of AGEs, oxidants, and markers of endothelial dysfunction throughout the study. The high-AGE meals before the supplement period had a profound effect on capillary blood flow in reaction to a stimulus, reducing it by 60%, while the ability of larger vessels to dilate (relax) in response to increased blood flow was diminished by 35%.50 These changes are typical of findings in people with early atherosclerosis, which can lead to heart attack or stroke.51-53

Remarkably, these detrimental effects on blood vessels were completely prevented by the three-day period of benfotiamine supplementation! Similarly, the AGE-induced post-meal increases in serum markers of endothelial dysfunction and oxidative stress were also significantly reduced after the supplement period. This landmark study, in the authors’ own words, “confirms micro- and macrovascular endothelial dysfunction accompanied by increased oxidative stress following a real-life, AGE-rich meal in individuals with type 2 diabetes and suggests benfotiamine as a potential treatment.”50 A follow-up study by the same research team late last year further showed that the vital molecule adiponectin—which promotes glucose uptake into skeletal muscle and increases fat burning54— decreases after a similar high-AGE meal. Again, benfotiamine prevented this detrimental effect of a meal rich in AGEs.55

Supplementing with Benfotiamine

Clinical studies to date suggest that the benefits of benfotiamine can be achieved at doses of 150-1,000 mg per day in divided doses. Benfotiamine appears to be highly safe, with no reports of toxicity or drug interactions in the scientific literature.56


We have come a long way in a few short years of nutritional research. Just over a decade ago, the devastating effects of AGE/RAGE reaction products on tissues were believed to be inevitable in people with diabetes, presenting the grim prospects of progressive loss of nerve, blood vessel, and kidney function, and their consequences on quality and quantity of life.3 Thanks to the discovery of the fat-soluble form of thiamine, benfotiamine, the past decade has witnessed an abundance of promising research to help dispel those ominous predictions and offers new hope for people with diabetes.7,14 Equally exciting work is now beginning to emerge, suggesting that this is only the beginning. Benfotiamine is currently being studied for its benefits in non-diabetic people as well, where it may help reduce DNA damage in people with end-stage renal disease,57 ensure protection from the endothelial dysfunction that leads to atherosclerosis and congestive heart failure, and provide relief in a host of additional painful nerve conditions.18 Benfotiamine’s impressive safety record supports its use in both diabetic and non-diabetic people interested in reducing the impact of AGE/RAGE on their longevity and quality of life.

If you have any questions on the scientific content of this article, please call a Life Extension Wellness Specialist at 1-800-226-2370.

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