Life Extension Magazine®
Age Reversal: An Unprecedented Research - Life Extension

Issue: March 2020

Age Reversal Update

An unprecedented human study aims to induce statistically significant and meaningful biological age reversal using multi-model interventions that include metformin, dasatinib, rapamycin, and NAD+ restoration therapy.

By William Faloon.

Each year I dedicate enormous time to a scientific conference titled Revolution Against Aging and Death.

The abbreviation for this event is “RAADfest.”

It is presented by a charitable group called the Coalition for Radical Life Extension.

The inspiration for RAADfest occurred in 2015, as a confluence of biomedical advances made clear that it may be possible to turn degenerative aging into a manageable condition in our lifetime.

Findings from small human studies that I help organize and fund, show improvements in clinical measures (such as blood pressure and joint function) and aging biomarkers (such as DNA health and inflammatory indicators).

I’ve self-experimented with many of these age reversal interventions.

So far, objective measures indicate that I may be growing biologically younger. The same may be true of most of the volunteers who participated in the proof-of-concept clinical trials.

The results of these studies are so impressive that a new charity was established in 2019 to fund a human age reversal trial that is unparalleled in the history of medical science.

The objective of this study is to carefully measure the effects of a combination of regenerative approaches that include:

  1. AMPK activation,
  2. Removal of senescent cells (using senolytics),
  3. Suppression of excess mTOR,
  4. Restoration of NAD+,
  5. Additional approaches being explored now.

This article will summarize a few of the major announcements made at RAADfest 2019. These include published results from a clinical trial that resulted in an unprecedented 2.5-year average reversal of biological age as measured by epigenetic aging clocks!

None of this would be possible without your purchases of Life Extension® nutrient formulas and laboratory tests.

Proceeds from these sales support scientific endeavors that have little or no commercial value but may enable substantive increases in healthy longevity to benefit all of humanity.

RAADfest 2019 may go down in the history books as a major inflection point in our battle against and victory over degenerative aging.

More than 260 people enrolled in a new project called the Vitality in Aging Longitudinal Study. This represented 25% of the 1,000 or so RAADfest 2019 attendees!

Even more would have enrolled if the Vitality in Aging research group had the time to expand its staff to accommodate the tremendous interest shown.

People had to be turned away from the clinic established in the hotel because the resources were not sufficient to enroll everyone who was eager to join.

The Vitality in Aging Longitudinal Study will enable meticulous measures of regenerative interventions, including the stair-step approach to biological age control outlined below.

Many more are expected to enroll as new clinical study sites open throughout the United States and beyond.

Human Age Reversal Demonstrated!

Interventions that may improve odds of living to year 2030

Unparalleled results from a published clinical trial were presented at RAADfest 2019.

The findings reveal a 2.5-year reversal in biological age markers in healthy men aged 51-65!

This study was conducted by Dr. Greg Fahy’s Intervene Immune group in collaboration with researchers from Stanford University and UCLA. This one-year treatment protocol showed:1

  1. Regenerated thymic structure,

  2. Improved immune function with signs of increased cancer protection,

  3. Improved prostate cancer markers (PSA and percent free PSA),

  4. Regenerative effects on kidney function and bone marrow, and

  5. 2.5 years of age reversal, on average, as measured by four different DNA methylation tests.

Biological age was assessed by multiple epigenetic aging clocks. Dr. Steve Horvath from UCLA, the original developer of this technology, who is considered to be the world’s foremost expert in its use, was one of the investigators in this trial.

Measurement of DNA methylation patterns may be the most accurate biomarker test to asses biological age and can be a strong predictor of future healthspan and lifespan.

The interventions used by Dr. Fahy’s group were individualized doses of metformin, DHEA, and human growth hormone.

The rationale for the study is that growth hormone is known to regenerate the thymus gland, which is vital for youthful immune responses. Downsides to growth hormone are insulin resistance and a potential to stimulate proliferation of malignant cells, but both problems were apparently contained by the protocol employed.

By combining metformin and DHEA with growth hormone for just one year, the benefits of growth hormone (such as systemic tissue regeneration) occurred with no significant side effects and the unexpected side benefit of an average of 2.5 years of biological age reversal as measured by DNA methylation clocks.

Before and after MRI tests validated the regeneration of thymic structure. (These MRI scans were funded in part by our Life Extension® group.)

And breaking news I just learned is that the study subjects that demonstrated 2.5 years of age reversal continue to demonstrate rejuvenating signs after the one year of low-dose growth hormone was discontinued.

Many of you already take DHEA as well as AMPK-activating compounds like metformin or AMPK-activating nutrients like Gynostemma pentaphyllum.

Dr. Fahy’s group at Intervene Immune is now launching an expanded study of this longevity protocol in Southern California and exploring ways to make treatment widely available and affordable. Additional information about their work appears on page 65 of this month’s issue.

My goal is to enable readers of Life Extension® magazine to gain access to this new age reversal intervention at ultra-low pricing as part of a large new study group managed by Intervene Immune.

Other potential longevity approaches were introduced at RAADfest 2019.

The Vitality in Aging Longitudinal Study looks forward to measuring the efficacy of these interventions and reporting the results to rapidly advance the science.

Enroll for RAADfest 2020 At Discount Prices

RAADfest 2019 attendees gained unexpected access to medical exams and advanced diagnostics at a fraction of normal pricing.

What’s more, a company that wants to support our studies committed to providing the initial study subjects with a complimentary bottle of dasatinib, a (senolytic drug), provided they have a prescription. This bottle of medication may last most of the early participants in the Vitality in Aging Longitudinal Study for several years. (New study subjects will have to purchase this medication, and the Age Reversal physicians’ network can refer them to low-cost sources.)

Most of the diagnostic tests performed at RAADfest 2019 will be repeated at RAADfest 2020 to see how much age reversal may have occurred in each study subject.

Large rooms have been reserved in the hotel to accommodate the expected number of people who want to enroll in clinical studies and initiate interventions at the RAADfest clinic, such as NAD+ infusions.

There has never been such an interactive conference where attendees not only learn about healthy longevity advances, but also get to participate as study subjects at the same time!

The potential value of having attended RAADfest 2019 cannot be overstated.

You can enroll now for RAADfest 2020 at a special rate of 15% off the current published price, which increases incrementally as we approach RAADfest.

Just log on to www.raadfest.com and use the discount code “LEF” when you register, in order to receive this special offer of 15% off.

This code is valid until March 1, 2020. To register at these lower prices, log on to: www.raadfest.com

P.S. The Age Reversal Network is an informal association, with no funding for staff. If you contact us with questions, please be patient with communication delays, as we have minimal staff to answer questions of a few thousand people currently registered to receive updates.

New Human Rejuvenation Trial

Those who enrolled in the Vitality in Aging Longitudinal Study are being carefully screened to meet eligibility criteria to enroll in the:

Vitality in Aging Interventions Trial

Vitality in Aging Interventions Trial Objectives

This clinical trial aims to induce statistically significant and meaningful age reversal in only 12 months. It will entail very close physician oversight and precise, careful introduction of the regenerative interventions.

Funding has been secured to study 40-50 people using multiple therapies and in-depth biomarkers to ascertain the degree of age reversal that may be occurring.

The interventions that will be introduced are represented by the first four steps in the image on the facing page to the left. Later Vitality in Aging studies will include newer interventions as cutting-edge research continues to provide more healthy longevity options.

New Donations Received

Humage Age Reversal Project

Right before my keynote RAADfest presentation, one of our supporters let me know that he was not happy about the progress made with a new charity I established called the Human Age Reversal Project.

Specifically, his concern was that as of October 1, 2019, we had only raised $395,000.

He made a new donation of more than $200,000. This, along with other donations received to date, brings our total to more than $675,000.

All Human Age Reversal Project donations are placed in an interest-bearing brokerage account. I expect this to grow much higher as more people understand the ramifications of this research for themselves and all of humanity.

I provided hundreds of thousands of dollars to launch the Vitality in Aging Longitudinal Study in 2019 and will continue to support it.

For the Vitality in Aging Interventions Trial, we will need new donations to expand beyond the first 15 months.

I ask that you help support the Human Age Reversal Project. We are accelerating age reversal initiatives and every penny goes towards the research; there are no salaries or fixed overhead costs!

Donation information is to the right:

The Medical Clinic at RAADfest

Bill Faloon speaking at RAADFest2019
Bill Faloon speaking at RAADFest

The medical clinic set up at RAADfest was filled with people seeking to enroll in the Vitality in Aging (VIA) Longitudinal Study.

Many who wanted to join could not because the lines were too long.

While huge resources were spent to open the Vitality in Aging Longitudinal Study clinic, the staff was unable to handle the unexpectedly large response.

Another temporary enrollment center was set up in Phoenix, Arizona, in November 2019 so that some of those who want to join this study could do so.

The Vitality in Aging research group spent three months after RAADfest 2019 processing applications, baseline clinical data, the many different blood samples taken, and enrolling more study subjects.

From the group of over 300 enrollees in the Vitality in Aging Longitudinal Study, an initial 40-50 will be selected who meet the eligibility criteria to qualify for the Vitality in Aging Interventions Study.

Just to clarify, before it can be determined if you meet the eligibility criteria to join the Vitality in Aging Interventions Study, you first need to enroll in the Vitality in Aging Longitudinal Study so your data can be collected and analyzed.

My RAADfest keynote talk introduced and explained the differences between these two human age reversal studies.

To view my 55-minute presentation (with new slides added), log on to the home page of the Age Reversal Network: www.age-reversal.net

About the Age Reversal Network (Age-Reversal.net)

Age Reversal Network may bring hope to your senior life

The purpose of the Age Reversal Network is to exchange scientific information, foster strategic alliances, and support biomedical endeavors aimed at reversing degenerative aging.

We seek to unite people in ways that will accelerate the availability of rejuvenation technologies to benefit all of humanity, including members of the group. As data emerge, the Age Reversal Network will seek to rapidly convey this to members of our private association.

The Age Reversal Network consists of several thousand individuals who have expressed their desire to donate to and/or actively participate in advancing human age reversal studies.

Our public benefit group functions as a private association and consists of physicians, scientists, activists, donors, and participants in previous age reversal initiatives. These individuals share a common desire to rejuvenate aged people.

Partnerships may form within or outside the group in any manner the individual members choose. Information will be shared at the discretion of the individual members.

The Age Reversal Network serves as an opensource communications channel to a wide variety of experimental technologies.

There are some individuals in this group who are bound by confidentiality/nondisclosure contracts. We nonetheless welcome their input and any meaningful scientific data they are permitted to disseminate. A key to our success will be opensource information sharing whenever feasible.

Those who choose to participate in clinical trials or self-experiment with therapies described by the Age Reversal Network should do so with the knowledge that any intervention can have unknown risks.

Members of this private association acknowledge they are embarking on a voyage with historic implications relating to human longevity. As with any exploratory venture, the outcome cannot be predicted, and any medical intervention carries inherent risks, especially for elderly individuals.

Professional medical advice should be sought before undergoing any potential treatment you learn about from the Age Reversal Network.

To register as a member of the Age Reversal Network and receive updates about regenerative medicine research initiatives, please log on to Age-Reversal.net/join. (There is no cost to join.)

Copyright © 2020 Age Reversal Network, All rights reserved.

How to Enroll in the Longitudinal Study

Medical Staff in Scrubs, Clapping at RAADFest 2020 Presentation

The Vitality in Aging Longitudinal Study is modeled after the famous Framingham Heart Study that enabled scientists to identify risk factors that cause heart attack and stroke.

Copied in these pages are Power Point slides I used at RAADfest to describe the Framingham Heart Study and how many lives were saved.

The plan is to rapidly identify risk factors that cause accelerated aging and what lifestyles and interventions (such as senolytic therapy) can reverse aging biomarkers.

Vitality in Aging Longitudinal Study sites are expected to open in most major cities. This will provide convenient access to people interested in joining a team effort to defeat aging.

The goal is to have qualified volunteer doctors in most major metropolitan areas, or you can travel to one of the study sites expected to open in Southern California, South Florida, Las Vegas, and Idaho.

The first step for those interested in participating in either of these studies is to have an Age Management Blood Test Panel. The results enable the research group and you to determine if you best qualify for either:

Vitality in Aging Longitudinal Study

Or

Vitality in Aging Interventions Trial

The retail price of the individual tests included in this new Age Management Blood Test Panel is around $3,000.

It is being made available for a limited time for $495, which includes your registration in the Vitality in Aging Longitudinal Study.

For those who utilize the Male or Female Blood Test Panels from LifeExtension®, the new Age Management Panel can be used to take its place with many more tests included.

The individual tests in the new Age Management Blood Test Panel can be viewed on the next page.

To order this blood test and enroll in the Vitality in Aging Longitudinal Study electronically, please log on to: VitalityInAging.org/sign-up

If you don’t want this blood test now, but want to be kept informed about the studies for your future consideration, please register your name and contact information at: VitalityInAging.org

By enrolling in the Longitudinal Study or registering your potential interest, you will be kept informed about new and current research initiatives spearheaded by the Vitality in Aging research group and local meetings.

To receive email updates about results from regenerative medicine trials, as well as about how you can gain affordable access to many of the interventions, you can join a private association called the Age Reversal Network at no cost by logging on to: https://age-reversal.net/join

Information on Vitality in Aging Trials

Concluding Remarks

doctor giving remarks at a conference

The Vitality in Aging research groups are making headway in measuring the efficacy of currently available regenerative interventions.

The goal is to identity which combinations of interventions are most effective in inducing meaningful human age reversal.

By enrolling in the Vitality in Aging Longitudinal Study, you obtain baseline measures of your state of health and can track improvements that occur in response to whichever rejuvenation protocol you choose (or do NOT choose).

Data sets will be compared to enable rapid identification of the most robust approaches to age reversal available today!

I look forward to keeping you posted on new plans for transforming emerging scientific findings into practical steps to improve your health and longevity.

To receive email updates about results from regenerative medicine trials, as well as about how you can gain affordable access, you can join a private association called the Age Reversal Network at no cost by logging on to: https://age-reversal.net/join

Note the Age Reversal Network is entirely separate from the Life Extension® group I founded in 1977 (and first published in 1980).

You can read a description of the Age Reversal Network below.

For longer life,

For Longer Life

William Faloon

Co-Founder,
Coalition for Radical Life Extension
Age Reversal Network


New Age Management Blood Panel

blood test tubes

The Vitality in Aging research group has come up with a blood test panel that provides keen insight into our “biological age,” along with affordability and ease of access.

The Age Management Blood Panel will enable as many people as possible to have these tests performed at baseline (before undergoing a potential age reversal intervention), and at follow-up intervals afterwards.

This new panel enables most to have a blood draw done in their local area. The results provide an in-depth snapshot of current health status to help determine what lifestyle changes or experimental interventions you may consider.

The cost of having all these tests done by commercial labs is outrageous. One large commercial lab quoted us over $3,000 for this elaborate test panel.

Through group purchasing, the Vitality in Aging research group has arranged for this comprehensive test panel to be available for $495, a savings of almost 85%.

This heavily subsidized price will be in effect only for a limited time. It will move higher as more people enroll and use up the subsidized pricing allotted for this study.

You can order the Age Management Blood Panel now and defer your blood draw until you are ready to initiate one of several experimental age reversal options. Current research protocol summaries are posted on https://vitalityinaging.org/via-interventions-trial/

To order the new Age Management Blood Test Panel, log on to VitalityInAging.org/sign-up

Here is a list of the tests included in the new Age Management Panel. Descriptions of the importance of some of the tests are provided below:

CARDIAC/INFLAMMATORY MARKERS

  • NMR Lipoprofile (measures atherogenic lipid risks + inflammation markers)
  • Homocysteine
  • C-Reactive Protein
  • IL-6 (interleukin-6)
  • Apolipoprotein B
  • Omega Index—Comprehensive (this is a finger stick kit)

HORMONES AND BINDING PROTEINS

  • IGF-1
  • IGF1BP3
  • DHEA-S
  • Free Testosterone
  • Total Testosterone
  • Estradiol
  • Progesterone (Female Only)
  • Vitamin D 25-Hydroxy

THYROID FUNCTION

  • Free T3
  • Free T4
  • TSH (Thyroid Stimulating Hormone)

INSULIN RESISTANCE MARKERS

  • Insulin
  • HbA1C
  • Ferritin

LIPIDS, GLUCOSE, LIVER-KIDNEY FUNCTION

  • Glucose
  • Uric Acid
  • BUN
  • Creatinine
  • eGFR
  • BUN/Creatinine
  • Sodium
  • Potassium
  • Chloride
  • Calcium
  • Phosphorus
  • Protein, Total
  • Albumin
  • Globulin
  • A/G Ratio
  • Bilirubin
  • Alkaline Phosphatase
  • LDH
  • AST
  • ALT
  • GGT
  • Iron
  • Cholesterol, Total
  • Triglycerides
  • HDL Cholesterol
  • LDL Cholesterol
  • T. Chol/HDL Ratio
  • Estimated CHD Risk

THE OMEGA-3 INDEX TEST MEASURES:

  • Omega-3 Index percent
  • Trans Fat Index
  • Omega-6:Omega-3 Ratio
  • AA:EPA Ratio
  • Full Fatty Acid Profile including:
  • Omega-3s
  • Omega-6s
  • Monounsaturated
  • Saturated
  • Trans

BLOOD COUNTS

  • WBC Count
  • RBC Count
  • Hemoglobin
  • Hematocrit
  • MCV
  • MCH
  • MCHC
  • RDW
  • Platelet count
  • Monocytes (% and absolute)
  • Eos (% and absolute)
  • Basos (% and absolute)
  • Neutrophils (% and absolute
  • Lymphs (% and absolute)
  • Immature granulocytes
  • Immature cells

PROSTATE CANCER SCREENING (MALES ONLY)

  • PSA–(Prostate-Specific Antigen)

Here is a description of some of the unique tests included in the Age Management Blood Test Panel:

  • IGF-1–(Insulin-like Growth Factor-1) is a surrogate marker of growth hormone status. Growth hormone is associated with muscle and skeletal strength, cognition, sleep, energy, and importantly, tissue recovery and repair. Too much growth hormone can create health issues just as too little can.

  • IGF1-BP3–(IGF Binding Protein 3)–This protein helps ensure IGF-1 is transported efficiently to tissues throughout the body. Both low and high levels may lead to concerns with IGF-1’s distribution and biological activity.

  • IL-6 (Interleukin-6)–A proinflammatory cytokine associated with chronic and acute inflammatory states. High levels may be associated with increased presence of senescent cells as well as many other degenerative and malignant health processes that may limit the efficacy of age reversal therapies. Long-lived individuals usually have low blood levels of IL-6.2-7 If your IL-6 is elevated, then measures will be recommended to reduce it.

  • Apolipoprotein B–A surface marker on non-HDL cholesterol, low blood levels of apolipoprotein B have been shown to be associated with decreased coronary artery disease risk by up to 90%.8 Another study shows regression of arterial plaque when apolipoprotein B blood levels are reduced.9

  • Omega-3 Index (complete)–An in-depth analysis of blood fatty acid profile, reflective of long-term dietary patterns and balance of proinflammatory and anti-inflammatory signaling molecule precursors. This comprehensive fatty acid profile includes the following individual tests:

    • Omega-3 Index–Overall percentage of omega-3 fatty acids in the blood. An optimal omega-3 index corresponds with positive cardiovascular, cognitive, and whole-body health and can be achieved through dietary consumption of omega-3-rich foods as well as with high-quality marine oil supplementation. Life Extension® recommends most people seek to achieve an Omega-3-Index score of 8% and higher.

    • Omega 6: Omega-3 ratio–Ratio of total omega-6 fatty acids compared to omega-3 fatty acids. To maintain healthy inflammatory response activity, Life Extension believes this ratio should remain under 4:1 and as close to 1:1 as possible.

    • AA:EPA ratio–A specific ratio of arachidonic acid (AA) and healthy eicosanoid promoting eicosapentaenoic acid (EPA). Ideal AA:EPA ratios are 1.7:1 or less.

    • Trans-Fat Index–Percentage of blood fatty acids in the unnatural trans-configuration, associated with higher processed food intake. Trans fats may interfere with the normal activity of natural fatty acids.

    • Full Fatty Acid Profile–Whole blood analysis of 24 fatty acids, including various polyunsaturated, monounsaturated, saturated, and trans-fatty acids. May help reveal irregular patterns of fatty acid conversion or imbalanced dietary fat intake.

  • NMR LipoProfile–A deep analysis of blood lipids, the NMR offers insight into LDL particle count, as well as average particle size (LDL pattern) providing better risk analysis of cholesterol lipoproteins and their relationship to atherogenesis. The NMR LipoProfile includes the following tests:

    • LDL particle number (LDL-P) -A direct measurement of LDL particle count, an independent risk factor for cardiovascular disease.

    • Small LDL particle number (small LDL-P)–Number of small, dense, LDL particles. Smaller LDL particles are considered more atherogenic, so higher levels are considered a cardiovascular risk factor.

    • HDL particle number (HDL-P)–A count of HDL particles, where higher levels are considered more protective, due to their role in reverse cholesterol transport.

    • LDL particle size–Pattern A is considered an ideal result, corresponding with larger, less atherogenic LDL particles.

    • Standard cholesterol test (LDL-C, HDL-C, triglycerides, and total cholesterol)—More predictive of risk than a simple total cholesterol test.

    • LP-IR (Insulin Resistance Score)–A composite score, based on various lipoprotein markers, that indicates risk of developing insulin resistance. Higher values indicate increased risk of developing insulin resistance.

Note: If the address you enter at (VIA blood test order landing page) is from one of the following states: NY, NJ, RI, MA, or MD, you will not be able to select “requisition” from the drop-down menu. If you have a residential address from one of the other 44 continental U.S. states, please enter that instead. Then you can drive to a LabCorp facility in any of the states other than the above-named five with the requisition that will be emailed to you, and have your blood drawn.

An alternative for those in the five states with restrictions would be to get a prescription from a doctor for the Age Management Panel. The much higher commercial lab price may make a blood draw trip to a neighboring state more cost effective.


References

  1. Fahy GM, Brooke RT, Watson JP, et al. Reversal of epigenetic aging and immunosenescent trends in humans. Aging Cell. 2019 Dec;18(6):e13028.
  2. Ershler WB, Keller ET. Age-associated increased interleukin-6 gene expression, late-life diseases, and frailty. Annu Rev Med. 2000;51:245-70.
  3. Harris TB, Ferrucci L, Tracy RP, et al. Associations of elevated interleukin-6 and C-reactive protein levels with mortality in the elderly. Am J Med. 1999 May;106(5):506-12.
  4. Duarte PO, Duarte MGF, Pelichek A, et al. Cardiovascular risk factors and inflammatory activity among centenarians with and without dementia. Aging Clin Exp Res. 2017 Jun;29(3):411-7.
  5. Garbers C, Kuck F, Aparicio-Siegmund S, et al. Cellular senescence or EGFR signaling induces Interleukin 6 (IL-6) receptor expression controlled by mammalian target of rapamycin (mTOR). Cell Cycle. 2013 Nov 1;12(21):3421-32.
  6. Gomez CR, Karavitis J, Palmer JL, et al. Interleukin-6 contributes to age-related alteration of cytokine production by macrophages. Mediators Inflamm. 2010;2010:475139.
  7. Varadhan R, Yao W, Matteini A, et al. Simple biologically informed inflammatory index of two serum cytokines predicts 10 year all-cause mortality in older adults. J Gerontol A Biol Sci Med Sci. 2014 Feb;69(2):165-73.
  8. Tabas I, Williams KJ, Boren J. Subendothelial lipoprotein retention as the initiating process in atherosclerosis: update and therapeutic implications. Circulation. 2007 Oct 16;116(16):1832-44.
  9. Masson W, Siniawski D, Lobo M, et al. Association between LDL-C, Non HDL-C, and Apolipoprotein B Levels with Coronary Plaque Regression. Arq Bras Cardiol. 2015 Jul;105(1):11-9.